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Objective To explore the predictive value of T cell activation in peripheral blood of patients with hepatocellular carcinoma(HCC) after anti PD-1 therapy and its ratio to tumor burden on the efficacy of immunotherapy. Methods Serum specimens were obtained before and after treatment from 85 patients with HCC who received anti-PD-1 treatment. Indicators such as cell subpopulations and T cell activation were detected by flow cytometry. Combined with imaging analysis, cutoff value was obtained by X-tile software. Survival analysis was used to evaluate patients' outcomes. Results The maximum fold change of Ki-67+/PD-1+/CD8+ T cells in treatment cycles and the tumor burden determined by imaging were associated with prognoses. The ratio of T cell Ki-67+/PD-1+/CD8+ expression to tumor burden ratio greater than 0.6 at the first cycle of anti-PD-1 immunotherapy was associated with improvements in progression-free survival and overall survival (P < 0.05). Conclusion The ratio of activationa in T cells in peripheral blood after immunotherapy to the tumor burden may be related to the clinical efficacy of anti-PD-1 immunotherapy for HCC.
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[Objective]To study effect of pain and stress response of NiTi rotary instrument for children with root canal therapy , and analyzed possible mechanisms.[Methods]60 cases(60 teeth)children with Dental pulp disease or root tip disease by root canal therapy from March 2015 to March 2017 were divided into observation group and control group. The control group used Hand NiTi en-larging file,observation group used NiTi rotary instruments,then root canal preparation,stress response,pain,filling effect and long-term efficacy were compared between two groups.[Result]The observation group root canal preparation time were significantly shorter than control group(P < 0.01),root canal deviation etc morphological changes(6.67%)was significantly lower than control group(26.67%,P < 0.05);gingival crevicular fluid TNF-α,IL-6,IL-1β were significantly lower than control group(P < 0.05, P < 0.01). In root canal preparation,after root canal preparation,pain rate were significantly lower than control group(10.00% vs 33.33%,3.33% vs 23.33%,P < 0.05);1 week after surgery,just fill rate(93.33%)was significantly higher than control group (70.00%,P<0.05). Followed up 6 months,root canal success rate(90.00%)was significantly higher than control group(66.67%, P<0.05).[Conclusion]NiTi rotary instruments help to reduce the pain in children with root canal,improve clinical efficacy,May be related to inhibition of surgical stress response.
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Objective To investigate the eating problems of outpatient infants,preschool age children(1 to 7 years old) enrolled in the Department of Child Health Care,Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University,and to analyze its correlation with children's physical development,so as to establish strategies for preventing abnormal eating habit in children.Methods A toll of 2458 children met the criteria,and caregivers (mothers) completed the Children's Eating Behavior Questionnaires (CEBQ) in Department of Child Health Care,Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University were selected and the children's sociodemographic data and the morbidity of children eating problems were investigated.The correlation between children's body mass index(BMI) with children's eating problems was determined by using Chi-square test and multiple regression analysis.Results About 66.2％ (1627/2458 cases) had normal weight,and 10.8％ (257/2458 cases) and 8.5 ％ (210/2458 cases) were overweight (BMI ＞ P85-P95) or obese (BMI ≥ P95),respectively.The prevalence of eating behavior problems was detected during 25-36 months.For 1-to-7-year-old children,the highest detection rate of eating problems was inattention and eating at non-permanent locations,occupying 64.7％ (1590/2458 cases)and 50.5％ (1241/2458 cases),respectively;the prevalence rate of preferring to junk food was the lowest,accounting for 19.3％ (474/2458 cases).The children's eating problems had a high association with the children's BMI.Among them,children with eating problems,such as difficultly in accepting the varying food stuff[at the age of 12 month,odds ratio(OR)=11.50,95％ confidence interval(CI):1.84-72.16] and eating at non-permanent locations(at 25-36month,OR=1.77,95 ％ CI:1.11-2.83),were prone to be wasting away;children with eating problems,such as preferring to junk food (at 12 month,OR=5.08,95 ％ CI:1.43-18.00;13-18 month,OR=2.17,95 ％ CI:1.06-4.44),rarely eating vegetables or fruit (19-24 month,OR=4.06,95％CI:1.46-11.31) and inattention (12 month,OR=3.85,95 ％ CI:1.52-9.79),were associated with overweight or obesity (all P＜0.01).Conclusions There was a high prevalence of eating problems in children between 12-84 month(1-7 years old) in Nanjing.Improper children's eating behaviors can increase the risks of wasting away or children's overweight/obesity.
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Objective To investigate correlation between cystatin C (Cys C) and target organ damage (TOD) in elderly hypertension and its clinical significance.Methods One hundred patients with TOD in elderly hypertension were selected as an observation group.One hundred patients without TOD in elderly hypertension were recruited as the controll group.And sixty healthy aged persons were recruited as healthy group.Serum concentration of Cys C in all cases was measured by the particle enhanced nephelometric immunoassay.At the same time,serum creatinine (Scr),urine albumin (mALB) and left ventricle mass index (LVMI) via ultrasonography were also detected.Correlation of Cys C with the above measurements were analyzed.Results The Cys C concentration was significantly higher in observation group [(1.84±0.32) mg/L] than in contrast [(0.92±0.36) mg/L] and healtbygroups [(0.85±0.34) mg/L],F=88.43,P=0.000.However,the difference in level of Cys C was not statistically significantly different between controll group and healthy group (P ＞0.05).In observation group,serum concentration of Cys C was positively correlated with Scr,mALBand LVMI (r=0.420,0.526,0.470,P=0.021,0.019,0.034) after adjusting age.Conclusions Serum level of Cys C is markedly elevated and is positively correlated with Scr,mALB and LVMI in patients with TOD in elderly hypertension.It can be considered as one of the useful factors for evaluating TOD in elderly hypertension.
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Objective To investigate the inlfuence of overexpression of miR-26b on the secretion of adipokines dur-ing human adipocyte differentiation. Methods Human preadipocytes were infected with the hsa-miR-26b over-expressing lentivirus and were induced to differentiate, and then the levels of adipokines (IL-6, leptin, resistin, TNF-α) at different time points during differentiation were measured by ELISA. Results Compared with control group, decreased secretions of both IL-6 and leptin, and increased secretion of resistin were found during the differentiation of human adipocytes in miR-26b overexpressed group. However, the secretion of TNF-αwas not measured in both groups. Conclusion The miR-26b can improve the inlfammation and insulin resistance of human adipocytes, which will provide potential targets for obesity treat-ment.
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Objective To predict the biological process and signaling pathways in which hsa-miR-1908 might be in-volved by a series of bioinformatics analysis, so as to lay foundations and provide theoretical basis for the further studies of hsa-miR-1908 biological function in human preadipocytes. Methods The sequence of hsa-miR-1908 was acquired from miR-Base database, and target genes of hsa-miR-1908 were predicted by miRanda, and then the intersection of the results and the results of gene-chip as gene set were further analyzed by gene ontology and pathway enrichment. Results The hsa-miR-1908 had some conserved property among different species. The functions of the target genes were enriched in Wnt receptor signal-ing pathway through beta-catenin, cell cycle, cell apeptosis and other biological processes. The GnRH signaling, MAPK sig-naling, insulin signaling, cell cycle signal transduction pathways and signaling pathway in pancreatic cancer were signiifcantly enriched. Conclusions The target genes set of hsa-miR-1908 were enriched in multiple biological process which are related with the obesity. This study provides guidance for the further study in human preadipocytes.
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Gastric cancer is the first in morbidity and mortality among cancers in China. The presence of peritoneal dissemination is the most common cause of recurrence after radical surgery, and has become a pivotal to the curative resection. Whether intraperitoneal chemotherapy can prevent peritoneal dissemination,lymph node metastasis, eradicate invisible seeding tumor cells, reduce peritoneal metastasis, improve survival and quality of life is always under investigation. This review focus on the theory, clinical applications and problems of intraperitoneal chemotherapy for gastric cancer.
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Objective To investigate the utility of full-field digital mammography (FFDM) and localization and biopsy in diagnosis of nonpalpable breast cancer. Methods 42 cases with suspect breast cancer by routine film reading but negative clinical palpation were performed localization and biopsy under X-ray orientation and diagnosed with breast cancer by pathology. The characters of those cases were studied.Results Among 42 cases, 5 cases showed micronodules, 8 cases demonstrated local dense infiltration, 11cases had architecture distortion, 18 cases demonstrated micro-calcifications. The type of pathology comprised of intraductal carcinoma (23 cases), tabular carcinoma in situ (2 cases), early infiltration of ductal carcinoma (12 cases), infiltrating ductal carcinoma (3 cases), papilloma carcinoma (2 cases). Conclusion FFDM can clearly show direct and indirect X-ray signs of breast cancer, combined with localization and biopsy the nonpalpable breast cancer can be easily diagnosed.
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Breast cancer is a systemic disease, only through multidisciplinary treatment including surgery, radiotherapy, chemotherapy, endocrine, can we enhance its effectiveness. In recent years, neoadjuvant chemotherapy for breast cancer has aroused great interest in tumor academia. We hope that this therapy can not only improve breast conservation rate, but also extend the overall survival through a series of clinical studies. This review presents the research progress of neoadjuvant chemotherapy for breast cancer.